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Selection and Characterization of DNA Aptamers for Egg White Lysozyme
We have selected aptamers binding to lysozyme from a DNA library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment. During the selection process the dissociation constant of the ssDNA pool decreased from the micromolar to the low nanomolar range within five ro...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257241/ https://www.ncbi.nlm.nih.gov/pubmed/20335968 http://dx.doi.org/10.3390/molecules15031127 |
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author | Tran, Dinh T. Janssen, Kris P. F. Pollet, Jeroen Lammertyn, Elke Anné, Jozef Van Schepdael, Ann Lammertyn, Jeroen |
author_facet | Tran, Dinh T. Janssen, Kris P. F. Pollet, Jeroen Lammertyn, Elke Anné, Jozef Van Schepdael, Ann Lammertyn, Jeroen |
author_sort | Tran, Dinh T. |
collection | PubMed |
description | We have selected aptamers binding to lysozyme from a DNA library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment. During the selection process the dissociation constant of the ssDNA pool decreased from the micromolar to the low nanomolar range within five rounds of selection. The final aptamer had a dissociation constant of 2.8 ± 0.3 nM, 6.1 ± 0.5 nM, and 52.9 ± 9.1 nM as determined by fluorescence anisotropy, surface plasmon resonance and affinity capillary electrophoresis respectively. The aptamers were successfully challenged for specificity against other egg white proteins. The high affinity aptamers open up possibilities for the development of aptamer based food and medical diagnostics. |
format | Online Article Text |
id | pubmed-6257241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-62572412018-12-04 Selection and Characterization of DNA Aptamers for Egg White Lysozyme Tran, Dinh T. Janssen, Kris P. F. Pollet, Jeroen Lammertyn, Elke Anné, Jozef Van Schepdael, Ann Lammertyn, Jeroen Molecules Article We have selected aptamers binding to lysozyme from a DNA library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment. During the selection process the dissociation constant of the ssDNA pool decreased from the micromolar to the low nanomolar range within five rounds of selection. The final aptamer had a dissociation constant of 2.8 ± 0.3 nM, 6.1 ± 0.5 nM, and 52.9 ± 9.1 nM as determined by fluorescence anisotropy, surface plasmon resonance and affinity capillary electrophoresis respectively. The aptamers were successfully challenged for specificity against other egg white proteins. The high affinity aptamers open up possibilities for the development of aptamer based food and medical diagnostics. Molecular Diversity Preservation International 2010-03-02 /pmc/articles/PMC6257241/ /pubmed/20335968 http://dx.doi.org/10.3390/molecules15031127 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Tran, Dinh T. Janssen, Kris P. F. Pollet, Jeroen Lammertyn, Elke Anné, Jozef Van Schepdael, Ann Lammertyn, Jeroen Selection and Characterization of DNA Aptamers for Egg White Lysozyme |
title | Selection and Characterization of DNA Aptamers for Egg White Lysozyme |
title_full | Selection and Characterization of DNA Aptamers for Egg White Lysozyme |
title_fullStr | Selection and Characterization of DNA Aptamers for Egg White Lysozyme |
title_full_unstemmed | Selection and Characterization of DNA Aptamers for Egg White Lysozyme |
title_short | Selection and Characterization of DNA Aptamers for Egg White Lysozyme |
title_sort | selection and characterization of dna aptamers for egg white lysozyme |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257241/ https://www.ncbi.nlm.nih.gov/pubmed/20335968 http://dx.doi.org/10.3390/molecules15031127 |
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