Pooled extracellular receptor-ligand interaction screening using CRISPR activation

Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-af...

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Detalles Bibliográficos
Autores principales: Chong, Zheng-Shan, Ohnishi, Shuhei, Yusa, Kosuke, Wright, Gavin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258485/
https://www.ncbi.nlm.nih.gov/pubmed/30477585
http://dx.doi.org/10.1186/s13059-018-1581-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258485/
https://www.ncbi.nlm.nih.gov/pubmed/30477585
http://dx.doi.org/10.1186/s13059-018-1581-3

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