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Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors
The antioxidant activity of the phenolic compounds present in industrial black liquors obtained from the two cooking processes (kraft and sulphite) used in Portugal to produce Eucalyptus globulus pulp was evaluated. The black liquors treated at several pH values were extracted with ethyl acetate. Ph...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259474/ https://www.ncbi.nlm.nih.gov/pubmed/21169882 http://dx.doi.org/10.3390/molecules15129308 |
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author | Faustino, Hélio Gil, Nuno Baptista, Cecília Duarte, Ana Paula |
author_facet | Faustino, Hélio Gil, Nuno Baptista, Cecília Duarte, Ana Paula |
author_sort | Faustino, Hélio |
collection | PubMed |
description | The antioxidant activity of the phenolic compounds present in industrial black liquors obtained from the two cooking processes (kraft and sulphite) used in Portugal to produce Eucalyptus globulus pulp was evaluated. The black liquors treated at several pH values were extracted with ethyl acetate. Phenolic fractions were further separated by liquid chromatography of the crude extracts of kraft liquor at pH = 6 and sulphite liquor at the original pH. Total phenolic content was determined in terms of gallic acid equivalents (Folin-Ciocalteu colorimetric method), and the antioxidant activity in the crude extracts at several pH values and in the separated fractions was measured using the DPPH test for radical scavenging capacity. The total phenolic content of crude extracts and separated fractions ranged from 92.7 to 181.6 and from 91.6 to 1,099.6 mg GAE/g, respectively, while the antioxidant activity index (AAI) ranged from 2.20 to 3.41 and from 2.21 to 11.47 respectively, showing very strong antioxidant activity in all studied cases. The fractions separated by column chromatography were submitted to mass spectrometry analysis and the results were compared to others in the literature of natural products, mainly from Eucalyptus, and the characteristic bands of functional groups were identified by (1)H-NMR and FTIR. These methods allowed the identification of 17 phenolic compounds. |
format | Online Article Text |
id | pubmed-6259474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62594742018-12-06 Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors Faustino, Hélio Gil, Nuno Baptista, Cecília Duarte, Ana Paula Molecules Article The antioxidant activity of the phenolic compounds present in industrial black liquors obtained from the two cooking processes (kraft and sulphite) used in Portugal to produce Eucalyptus globulus pulp was evaluated. The black liquors treated at several pH values were extracted with ethyl acetate. Phenolic fractions were further separated by liquid chromatography of the crude extracts of kraft liquor at pH = 6 and sulphite liquor at the original pH. Total phenolic content was determined in terms of gallic acid equivalents (Folin-Ciocalteu colorimetric method), and the antioxidant activity in the crude extracts at several pH values and in the separated fractions was measured using the DPPH test for radical scavenging capacity. The total phenolic content of crude extracts and separated fractions ranged from 92.7 to 181.6 and from 91.6 to 1,099.6 mg GAE/g, respectively, while the antioxidant activity index (AAI) ranged from 2.20 to 3.41 and from 2.21 to 11.47 respectively, showing very strong antioxidant activity in all studied cases. The fractions separated by column chromatography were submitted to mass spectrometry analysis and the results were compared to others in the literature of natural products, mainly from Eucalyptus, and the characteristic bands of functional groups were identified by (1)H-NMR and FTIR. These methods allowed the identification of 17 phenolic compounds. MDPI 2010-12-16 /pmc/articles/PMC6259474/ /pubmed/21169882 http://dx.doi.org/10.3390/molecules15129308 Text en © 2010 by the authors; http://creativecommons.org/licenses/by/3.0/ licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Faustino, Hélio Gil, Nuno Baptista, Cecília Duarte, Ana Paula Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors |
title | Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors |
title_full | Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors |
title_fullStr | Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors |
title_full_unstemmed | Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors |
title_short | Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors |
title_sort | antioxidant activity of lignin phenolic compounds extracted from kraft and sulphite black liquors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259474/ https://www.ncbi.nlm.nih.gov/pubmed/21169882 http://dx.doi.org/10.3390/molecules15129308 |
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