Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262049/ https://www.ncbi.nlm.nih.gov/pubmed/30524415 http://dx.doi.org/10.3389/fmicb.2018.02853 |
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author | Sekizuka, Tsuyoshi Matsui, Mari Takahashi, Tomiyo Hayashi, Michiko Suzuki, Satowa Tokaji, Akihiko Kuroda, Makoto |
author_facet | Sekizuka, Tsuyoshi Matsui, Mari Takahashi, Tomiyo Hayashi, Michiko Suzuki, Satowa Tokaji, Akihiko Kuroda, Makoto |
author_sort | Sekizuka, Tsuyoshi |
collection | PubMed |
description | A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that it could be novel species, most similar to [Kluyvera] intestini, of which taxonomic nomenclature is still under discussion. Orthologous conserved gene analysis among 42 related bacterial strains indicated that MRY16-398 was classified as the newly proposed genus Metakosakonia. Further, MRY16-398 was found to harbor the bla(IMP-6) gene-positive class 1 integron (In722) in plasmid pMRY16-398_2 (IncN replicon, 47.4 kb in size). This finding implies that rare and opportunistic bacteria could be potential infectious agents. In conclusion, our results highlight the need for continuous monitoring for CPE even in nonpathogenic bacteria in the nosocomial environment. |
format | Online Article Text |
id | pubmed-6262049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62620492018-12-06 Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient Sekizuka, Tsuyoshi Matsui, Mari Takahashi, Tomiyo Hayashi, Michiko Suzuki, Satowa Tokaji, Akihiko Kuroda, Makoto Front Microbiol Microbiology A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that it could be novel species, most similar to [Kluyvera] intestini, of which taxonomic nomenclature is still under discussion. Orthologous conserved gene analysis among 42 related bacterial strains indicated that MRY16-398 was classified as the newly proposed genus Metakosakonia. Further, MRY16-398 was found to harbor the bla(IMP-6) gene-positive class 1 integron (In722) in plasmid pMRY16-398_2 (IncN replicon, 47.4 kb in size). This finding implies that rare and opportunistic bacteria could be potential infectious agents. In conclusion, our results highlight the need for continuous monitoring for CPE even in nonpathogenic bacteria in the nosocomial environment. Frontiers Media S.A. 2018-11-22 /pmc/articles/PMC6262049/ /pubmed/30524415 http://dx.doi.org/10.3389/fmicb.2018.02853 Text en Copyright © 2018 Sekizuka, Matsui, Takahashi, Hayashi, Suzuki, Tokaji and Kuroda. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Sekizuka, Tsuyoshi Matsui, Mari Takahashi, Tomiyo Hayashi, Michiko Suzuki, Satowa Tokaji, Akihiko Kuroda, Makoto Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient |
title | Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient |
title_full | Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient |
title_fullStr | Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient |
title_full_unstemmed | Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient |
title_short | Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient |
title_sort | complete genome sequence of bla(imp–6)-positive metakosakonia sp. mry16-398 isolate from the ascites of a diverticulitis patient |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262049/ https://www.ncbi.nlm.nih.gov/pubmed/30524415 http://dx.doi.org/10.3389/fmicb.2018.02853 |
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