Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient

A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that i...

Descripción completa

Detalles Bibliográficos
Autores principales: Sekizuka, Tsuyoshi, Matsui, Mari, Takahashi, Tomiyo, Hayashi, Michiko, Suzuki, Satowa, Tokaji, Akihiko, Kuroda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262049/
https://www.ncbi.nlm.nih.gov/pubmed/30524415
http://dx.doi.org/10.3389/fmicb.2018.02853
_version_ 1783375034170998784
author Sekizuka, Tsuyoshi
Matsui, Mari
Takahashi, Tomiyo
Hayashi, Michiko
Suzuki, Satowa
Tokaji, Akihiko
Kuroda, Makoto
author_facet Sekizuka, Tsuyoshi
Matsui, Mari
Takahashi, Tomiyo
Hayashi, Michiko
Suzuki, Satowa
Tokaji, Akihiko
Kuroda, Makoto
author_sort Sekizuka, Tsuyoshi
collection PubMed
description A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that it could be novel species, most similar to [Kluyvera] intestini, of which taxonomic nomenclature is still under discussion. Orthologous conserved gene analysis among 42 related bacterial strains indicated that MRY16-398 was classified as the newly proposed genus Metakosakonia. Further, MRY16-398 was found to harbor the bla(IMP-6) gene-positive class 1 integron (In722) in plasmid pMRY16-398_2 (IncN replicon, 47.4 kb in size). This finding implies that rare and opportunistic bacteria could be potential infectious agents. In conclusion, our results highlight the need for continuous monitoring for CPE even in nonpathogenic bacteria in the nosocomial environment.
format Online
Article
Text
id pubmed-6262049
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-62620492018-12-06 Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient Sekizuka, Tsuyoshi Matsui, Mari Takahashi, Tomiyo Hayashi, Michiko Suzuki, Satowa Tokaji, Akihiko Kuroda, Makoto Front Microbiol Microbiology A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that it could be novel species, most similar to [Kluyvera] intestini, of which taxonomic nomenclature is still under discussion. Orthologous conserved gene analysis among 42 related bacterial strains indicated that MRY16-398 was classified as the newly proposed genus Metakosakonia. Further, MRY16-398 was found to harbor the bla(IMP-6) gene-positive class 1 integron (In722) in plasmid pMRY16-398_2 (IncN replicon, 47.4 kb in size). This finding implies that rare and opportunistic bacteria could be potential infectious agents. In conclusion, our results highlight the need for continuous monitoring for CPE even in nonpathogenic bacteria in the nosocomial environment. Frontiers Media S.A. 2018-11-22 /pmc/articles/PMC6262049/ /pubmed/30524415 http://dx.doi.org/10.3389/fmicb.2018.02853 Text en Copyright © 2018 Sekizuka, Matsui, Takahashi, Hayashi, Suzuki, Tokaji and Kuroda. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sekizuka, Tsuyoshi
Matsui, Mari
Takahashi, Tomiyo
Hayashi, Michiko
Suzuki, Satowa
Tokaji, Akihiko
Kuroda, Makoto
Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
title Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
title_full Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
title_fullStr Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
title_full_unstemmed Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
title_short Complete Genome Sequence of bla(IMP–6)-Positive Metakosakonia sp. MRY16-398 Isolate From the Ascites of a Diverticulitis Patient
title_sort complete genome sequence of bla(imp–6)-positive metakosakonia sp. mry16-398 isolate from the ascites of a diverticulitis patient
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262049/
https://www.ncbi.nlm.nih.gov/pubmed/30524415
http://dx.doi.org/10.3389/fmicb.2018.02853
work_keys_str_mv AT sekizukatsuyoshi completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient
AT matsuimari completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient
AT takahashitomiyo completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient
AT hayashimichiko completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient
AT suzukisatowa completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient
AT tokajiakihiko completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient
AT kurodamakoto completegenomesequenceofblaimp6positivemetakosakoniaspmry16398isolatefromtheascitesofadiverticulitispatient