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High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation

Since the initial description of phage display technology for the generation of human antibodies, a variety of selection methods has been developed. The most critical parameter for all in vitro-based approaches is the quality of the antibody library. Concurrent evolution of the libraries has allowed...

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Autores principales: Ponsel, Dirk, Neugebauer, Julia, Ladetzki-Baehs, Kathrin, Tissot, Kathrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263270/
https://www.ncbi.nlm.nih.gov/pubmed/21540796
http://dx.doi.org/10.3390/molecules16053675
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author Ponsel, Dirk
Neugebauer, Julia
Ladetzki-Baehs, Kathrin
Tissot, Kathrin
author_facet Ponsel, Dirk
Neugebauer, Julia
Ladetzki-Baehs, Kathrin
Tissot, Kathrin
author_sort Ponsel, Dirk
collection PubMed
description Since the initial description of phage display technology for the generation of human antibodies, a variety of selection methods has been developed. The most critical parameter for all in vitro-based approaches is the quality of the antibody library. Concurrent evolution of the libraries has allowed display and selection technologies to reveal their full potential. They come in different flavors, from naïve to fully synthetic and differ in terms of size, quality, method of preparation, framework and CDR composition. Early on, the focus has mainly been on affinities and thus on library size and diversity. Subsequently, the increased awareness of developability and cost of goods as important success factors has spurred efforts to generate libraries with improved biophysical properties and favorable production characteristics. More recently a major focus on reduction of unwanted side effects through reduced immunogenicity and improved overall biophysical behavior has led to a re-evaluation of library design.
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spelling pubmed-62632702018-12-10 High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation Ponsel, Dirk Neugebauer, Julia Ladetzki-Baehs, Kathrin Tissot, Kathrin Molecules Review Since the initial description of phage display technology for the generation of human antibodies, a variety of selection methods has been developed. The most critical parameter for all in vitro-based approaches is the quality of the antibody library. Concurrent evolution of the libraries has allowed display and selection technologies to reveal their full potential. They come in different flavors, from naïve to fully synthetic and differ in terms of size, quality, method of preparation, framework and CDR composition. Early on, the focus has mainly been on affinities and thus on library size and diversity. Subsequently, the increased awareness of developability and cost of goods as important success factors has spurred efforts to generate libraries with improved biophysical properties and favorable production characteristics. More recently a major focus on reduction of unwanted side effects through reduced immunogenicity and improved overall biophysical behavior has led to a re-evaluation of library design. MDPI 2011-05-03 /pmc/articles/PMC6263270/ /pubmed/21540796 http://dx.doi.org/10.3390/molecules16053675 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Ponsel, Dirk
Neugebauer, Julia
Ladetzki-Baehs, Kathrin
Tissot, Kathrin
High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
title High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
title_full High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
title_fullStr High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
title_full_unstemmed High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
title_short High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
title_sort high affinity, developability and functional size: the holy grail of combinatorial antibody library generation
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263270/
https://www.ncbi.nlm.nih.gov/pubmed/21540796
http://dx.doi.org/10.3390/molecules16053675
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