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Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects

The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatog...

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Autores principales: Liu, Xian Qiong, Wu, Hao, Yu, Hong Li, Zhao, Teng Fei, Pan, Yao Zong, Shi, Run Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264375/
https://www.ncbi.nlm.nih.gov/pubmed/22083235
http://dx.doi.org/10.3390/molecules16119480
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author Liu, Xian Qiong
Wu, Hao
Yu, Hong Li
Zhao, Teng Fei
Pan, Yao Zong
Shi, Run Jun
author_facet Liu, Xian Qiong
Wu, Hao
Yu, Hong Li
Zhao, Teng Fei
Pan, Yao Zong
Shi, Run Jun
author_sort Liu, Xian Qiong
collection PubMed
description The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 μg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 μg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 μg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E(2) (PGE(2)) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the number of resident cells following peritoneal lavage did not reduce the AEL-induced neutrophil migration. However, pre-treatment with 3% thioglycollate which increased the peritoneal macrophage population by 201%, enhanced the neutrophil migration induced by AEL (200 μg/mL/cavity) (p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of rat peritoneal cavities with compound 48/80 (N-methyl-p-methoxyphenethylamine with formaldehyde) did not modify AEL-induced neutrophil migration. The results provided the basis for identifying the toxic components of A. erubescens and AEL could be a new useful tool for pro-inflammatory research.
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spelling pubmed-62643752018-12-10 Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects Liu, Xian Qiong Wu, Hao Yu, Hong Li Zhao, Teng Fei Pan, Yao Zong Shi, Run Jun Molecules Article The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 μg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 μg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 μg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E(2) (PGE(2)) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the number of resident cells following peritoneal lavage did not reduce the AEL-induced neutrophil migration. However, pre-treatment with 3% thioglycollate which increased the peritoneal macrophage population by 201%, enhanced the neutrophil migration induced by AEL (200 μg/mL/cavity) (p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of rat peritoneal cavities with compound 48/80 (N-methyl-p-methoxyphenethylamine with formaldehyde) did not modify AEL-induced neutrophil migration. The results provided the basis for identifying the toxic components of A. erubescens and AEL could be a new useful tool for pro-inflammatory research. MDPI 2011-11-14 /pmc/articles/PMC6264375/ /pubmed/22083235 http://dx.doi.org/10.3390/molecules16119480 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liu, Xian Qiong
Wu, Hao
Yu, Hong Li
Zhao, Teng Fei
Pan, Yao Zong
Shi, Run Jun
Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects
title Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects
title_full Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects
title_fullStr Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects
title_full_unstemmed Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects
title_short Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects
title_sort purification of a lectin from arisaema erubescens (wall.) schott and its pro-inflammatory effects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264375/
https://www.ncbi.nlm.nih.gov/pubmed/22083235
http://dx.doi.org/10.3390/molecules16119480
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