Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection

Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and for isolation of specific cell populations for study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However,...

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Detalles Bibliográficos
Autores principales: Sluch, Valentin M., Chamling, Xitiz, Wenger, Claire, Duan, Yukan, Rice, Dennis S., Zack, Donald J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264506/
https://www.ncbi.nlm.nih.gov/pubmed/30496180
http://dx.doi.org/10.1371/journal.pone.0201683

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264506/
https://www.ncbi.nlm.nih.gov/pubmed/30496180
http://dx.doi.org/10.1371/journal.pone.0201683

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