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Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides

A thermostable β-1,3-galactosidase from Marinomonas sp. BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of IPTG to the medium when the OD(600) of the culture rea...

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Detalles Bibliográficos
Autores principales: Ding, Haitao, Zhou, Lili, Zeng, Qian, Yu, Yong, Chen, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267478/
https://www.ncbi.nlm.nih.gov/pubmed/30380738
http://dx.doi.org/10.3390/md16110415