Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluore...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268487/ https://www.ncbi.nlm.nih.gov/pubmed/22382526 http://dx.doi.org/10.3390/molecules17032474 |
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author | Jun, Bong-Hyun Kang, Homan Lee, Yoon-Sik Jeong, Dae Hong |
author_facet | Jun, Bong-Hyun Kang, Homan Lee, Yoon-Sik Jeong, Dae Hong |
author_sort | Jun, Bong-Hyun |
collection | PubMed |
description | Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding. |
format | Online Article Text |
id | pubmed-6268487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62684872018-12-20 Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads Jun, Bong-Hyun Kang, Homan Lee, Yoon-Sik Jeong, Dae Hong Molecules Review Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding. MDPI 2012-03-01 /pmc/articles/PMC6268487/ /pubmed/22382526 http://dx.doi.org/10.3390/molecules17032474 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Jun, Bong-Hyun Kang, Homan Lee, Yoon-Sik Jeong, Dae Hong Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads |
title | Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads |
title_full | Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads |
title_fullStr | Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads |
title_full_unstemmed | Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads |
title_short | Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads |
title_sort | fluorescence-based multiplex protein detection using optically encoded microbeads |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268487/ https://www.ncbi.nlm.nih.gov/pubmed/22382526 http://dx.doi.org/10.3390/molecules17032474 |
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