Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluore...

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Autores principales: Jun, Bong-Hyun, Kang, Homan, Lee, Yoon-Sik, Jeong, Dae Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268487/
https://www.ncbi.nlm.nih.gov/pubmed/22382526
http://dx.doi.org/10.3390/molecules17032474
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author Jun, Bong-Hyun
Kang, Homan
Lee, Yoon-Sik
Jeong, Dae Hong
author_facet Jun, Bong-Hyun
Kang, Homan
Lee, Yoon-Sik
Jeong, Dae Hong
author_sort Jun, Bong-Hyun
collection PubMed
description Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
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spelling pubmed-62684872018-12-20 Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads Jun, Bong-Hyun Kang, Homan Lee, Yoon-Sik Jeong, Dae Hong Molecules Review Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding. MDPI 2012-03-01 /pmc/articles/PMC6268487/ /pubmed/22382526 http://dx.doi.org/10.3390/molecules17032474 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Jun, Bong-Hyun
Kang, Homan
Lee, Yoon-Sik
Jeong, Dae Hong
Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
title Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
title_full Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
title_fullStr Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
title_full_unstemmed Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
title_short Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
title_sort fluorescence-based multiplex protein detection using optically encoded microbeads
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268487/
https://www.ncbi.nlm.nih.gov/pubmed/22382526
http://dx.doi.org/10.3390/molecules17032474
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