Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence

A novel phosphoramidite derivative of cholesterol, with an ether-linked hexaethylene glycol (HEG) spacer arm, has been obtained through simple and reproducible solid phase modified oligonucleotide synthesis manipulations. This building block and the known phosphoramidite derivative of 3β-(2-hydroxye...

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Autores principales: Musumeci, Domenica, Montesarchio, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268758/
https://www.ncbi.nlm.nih.gov/pubmed/23090019
http://dx.doi.org/10.3390/molecules171012378
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author Musumeci, Domenica
Montesarchio, Daniela
author_facet Musumeci, Domenica
Montesarchio, Daniela
author_sort Musumeci, Domenica
collection PubMed
description A novel phosphoramidite derivative of cholesterol, with an ether-linked hexaethylene glycol (HEG) spacer arm, has been obtained through simple and reproducible solid phase modified oligonucleotide synthesis manipulations. This building block and the known phosphoramidite derivative of 3β-(2-hydroxyethoxy)cholesterol have been exploited in standard oligonucleotide synthesis protocols for the preparation of 5'- conjugates of the G-quadruplex-forming (5)(')TGGGAG(3)(') oligomer, known as the Hotoda’s sequence, to produce new potential anti-HIV agents.
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spelling pubmed-62687582018-12-12 Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence Musumeci, Domenica Montesarchio, Daniela Molecules Article A novel phosphoramidite derivative of cholesterol, with an ether-linked hexaethylene glycol (HEG) spacer arm, has been obtained through simple and reproducible solid phase modified oligonucleotide synthesis manipulations. This building block and the known phosphoramidite derivative of 3β-(2-hydroxyethoxy)cholesterol have been exploited in standard oligonucleotide synthesis protocols for the preparation of 5'- conjugates of the G-quadruplex-forming (5)(')TGGGAG(3)(') oligomer, known as the Hotoda’s sequence, to produce new potential anti-HIV agents. MDPI 2012-10-22 /pmc/articles/PMC6268758/ /pubmed/23090019 http://dx.doi.org/10.3390/molecules171012378 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Musumeci, Domenica
Montesarchio, Daniela
Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence
title Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence
title_full Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence
title_fullStr Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence
title_full_unstemmed Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence
title_short Synthesis of a Cholesteryl-HEG Phosphoramidite Derivative and Its Application to Lipid-conjugates of the Anti-HIV (5')TGGGAG(3') Hotoda’s Sequence
title_sort synthesis of a cholesteryl-heg phosphoramidite derivative and its application to lipid-conjugates of the anti-hiv (5')tgggag(3') hotoda’s sequence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268758/
https://www.ncbi.nlm.nih.gov/pubmed/23090019
http://dx.doi.org/10.3390/molecules171012378
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