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Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides

We demonstrated that 5-vinyldeoxyuridine ((V)U) and 5-carboxyvinyldeoxyuridine ((CV)U) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any...

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Detalles Bibliográficos
Autores principales: Nakamura, Shigetaka, Ogasawara, Shinzi, Matuda, Shigeo, Saito, Isao, Fujimoto, Kenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268978/
https://www.ncbi.nlm.nih.gov/pubmed/22202805
http://dx.doi.org/10.3390/molecules17010163