The Application of Transient-State Kinetic Isotope Effects to the Resolution of Mechanisms of Enzyme-Catalyzed Reactions

Much of our understanding of the mechanisms of enzyme-catalyzed reactions is based on steady-state kinetic studies. Experimentally, this approach depends solely on the measurement of rates of free product appearance (d[P]/dt), a mechanistically and mathematically complex entity. Despite the ambiguit...

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Detalles Bibliográficos
Autor principal: Fisher, Harvey F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269668/
https://www.ncbi.nlm.nih.gov/pubmed/23857126
http://dx.doi.org/10.3390/molecules18078230

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269668/
https://www.ncbi.nlm.nih.gov/pubmed/23857126
http://dx.doi.org/10.3390/molecules18078230

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