A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors

An assay for telomerase activity based on asymmetric polymerase chain reaction (A-PCR) on magnetic beads (MBs) and subsequent application of cycling probe technology (CPT) is described. In this assay, the telomerase reaction products are immobilized on MBs, which are then washed to remove PCR inhibi...

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Detalles Bibliográficos
Autores principales: Yaku, Hidenobu, Murashima, Takashi, Miyoshi, Daisuke, Sugimoto, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269933/
https://www.ncbi.nlm.nih.gov/pubmed/24071983
http://dx.doi.org/10.3390/molecules181011751

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269933/
https://www.ncbi.nlm.nih.gov/pubmed/24071983
http://dx.doi.org/10.3390/molecules181011751

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