Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand

Macrocyclic hexaoxazole dimer of L2H2-6OTD-dimer (3) was newly synthesized as a telomeric G-quadruplex (G4) ligand, and interaction with long telomeric DNAs telo48, 72, and 96 was evaluated by means of electrophoresis mobility shift assay, CD spectra analysis, and CD melting experiments. The L2H2-6O...

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Autores principales: Iida, Keisuke, Majima, Satoki, Nakamura, Takahiro, Seimiya, Hiroyuki, Nagasawa, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269967/
https://www.ncbi.nlm.nih.gov/pubmed/23584054
http://dx.doi.org/10.3390/molecules18044328
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author Iida, Keisuke
Majima, Satoki
Nakamura, Takahiro
Seimiya, Hiroyuki
Nagasawa, Kazuo
author_facet Iida, Keisuke
Majima, Satoki
Nakamura, Takahiro
Seimiya, Hiroyuki
Nagasawa, Kazuo
author_sort Iida, Keisuke
collection PubMed
description Macrocyclic hexaoxazole dimer of L2H2-6OTD-dimer (3) was newly synthesized as a telomeric G-quadruplex (G4) ligand, and interaction with long telomeric DNAs telo48, 72, and 96 was evaluated by means of electrophoresis mobility shift assay, CD spectra analysis, and CD melting experiments. The L2H2-6OTD-dimer (3) interacted with the long telomeric DNAs by inducing anti-parallel type G4 structure of each unit of 24 bases, i.e., (TTAGGG)(4) sequences. Dimer 3 stabilizes long telomeric DNAs more efficiently than the corresponding monomer of L2H2-6OTD (2). It showed potent inhibitory activity against telomerase, with an IC(50) value of 7.5 nm.
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spelling pubmed-62699672018-12-14 Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand Iida, Keisuke Majima, Satoki Nakamura, Takahiro Seimiya, Hiroyuki Nagasawa, Kazuo Molecules Article Macrocyclic hexaoxazole dimer of L2H2-6OTD-dimer (3) was newly synthesized as a telomeric G-quadruplex (G4) ligand, and interaction with long telomeric DNAs telo48, 72, and 96 was evaluated by means of electrophoresis mobility shift assay, CD spectra analysis, and CD melting experiments. The L2H2-6OTD-dimer (3) interacted with the long telomeric DNAs by inducing anti-parallel type G4 structure of each unit of 24 bases, i.e., (TTAGGG)(4) sequences. Dimer 3 stabilizes long telomeric DNAs more efficiently than the corresponding monomer of L2H2-6OTD (2). It showed potent inhibitory activity against telomerase, with an IC(50) value of 7.5 nm. MDPI 2013-04-12 /pmc/articles/PMC6269967/ /pubmed/23584054 http://dx.doi.org/10.3390/molecules18044328 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Iida, Keisuke
Majima, Satoki
Nakamura, Takahiro
Seimiya, Hiroyuki
Nagasawa, Kazuo
Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand
title Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand
title_full Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand
title_fullStr Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand
title_full_unstemmed Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand
title_short Evaluation of the Interaction between Long Telomeric DNA and Macrocyclic Hexaoxazole (6OTD) Dimer of a G-quadruplex Ligand
title_sort evaluation of the interaction between long telomeric dna and macrocyclic hexaoxazole (6otd) dimer of a g-quadruplex ligand
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269967/
https://www.ncbi.nlm.nih.gov/pubmed/23584054
http://dx.doi.org/10.3390/molecules18044328
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