Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target an...

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Detalles Bibliográficos
Autores principales: Ito, Kenichiro, Liu, Hongshan, Komiyama, Makoto, Hayashi, Tetsuya, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269988/
https://www.ncbi.nlm.nih.gov/pubmed/24141242
http://dx.doi.org/10.3390/molecules181012909

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269988/
https://www.ncbi.nlm.nih.gov/pubmed/24141242
http://dx.doi.org/10.3390/molecules181012909

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