Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target an...

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Detalles Bibliográficos
Autores principales: Ito, Kenichiro, Liu, Hongshan, Komiyama, Makoto, Hayashi, Tetsuya, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269988/
https://www.ncbi.nlm.nih.gov/pubmed/24141242
http://dx.doi.org/10.3390/molecules181012909
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author Ito, Kenichiro
Liu, Hongshan
Komiyama, Makoto
Hayashi, Tetsuya
Xu, Yan
author_facet Ito, Kenichiro
Liu, Hongshan
Komiyama, Makoto
Hayashi, Tetsuya
Xu, Yan
author_sort Ito, Kenichiro
collection PubMed
description 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.
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spelling pubmed-62699882018-12-18 Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions Ito, Kenichiro Liu, Hongshan Komiyama, Makoto Hayashi, Tetsuya Xu, Yan Molecules Article 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells. MDPI 2013-10-17 /pmc/articles/PMC6269988/ /pubmed/24141242 http://dx.doi.org/10.3390/molecules181012909 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ito, Kenichiro
Liu, Hongshan
Komiyama, Makoto
Hayashi, Tetsuya
Xu, Yan
Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
title Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
title_full Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
title_fullStr Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
title_full_unstemmed Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
title_short Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
title_sort direct light-up of camp derivatives in living cells by click reactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269988/
https://www.ncbi.nlm.nih.gov/pubmed/24141242
http://dx.doi.org/10.3390/molecules181012909
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AT hayashitetsuya directlightupofcampderivativesinlivingcellsbyclickreactions
AT xuyan directlightupofcampderivativesinlivingcellsbyclickreactions

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