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Comparative Analysis of Click Chemistry Mediated Activity-Based Protein Profiling in Cell Lysates
Activity-based protein profiling uses chemical probes that covalently attach to active enzyme targets. Probes with conventional tags have disadvantages, such as limited cell permeability or steric hindrance around the reactive group. A tandem labeling strategy with click chemistry is now widely used...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270401/ https://www.ncbi.nlm.nih.gov/pubmed/24126377 http://dx.doi.org/10.3390/molecules181012599 |