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ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl(2)] (1) and [Cu(H2BzMe)Cl(2)] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 pro...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270821/ https://www.ncbi.nlm.nih.gov/pubmed/25350363 http://dx.doi.org/10.3390/molecules191117202 |
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author | Despaigne, Angel A. Recio Da Silva, Jeferson G. da Costa, Pryscila R. dos Santos, Raquel G. Beraldo, Heloisa |
author_facet | Despaigne, Angel A. Recio Da Silva, Jeferson G. da Costa, Pryscila R. dos Santos, Raquel G. Beraldo, Heloisa |
author_sort | Despaigne, Angel A. Recio |
collection | PubMed |
description | 2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl(2)] (1) and [Cu(H2BzMe)Cl(2)] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 proved to be more active than the corresponding hydrazones against U87, but not against T98 cells. Compound 1 induced higher levels of ROS than H2AcMe in both glioma cell lines. H2AcMe and 1 induced lower levels of ROS in MRC5 than in U87 cells. Compound 2 induced lower levels of ROS in MRC5 than in T98 cells. The cytotoxic effect of 1 in U87 cells could be related to its ability to provoke the release of ROS, suggesting that the cytotoxicity of 1 might be somehow p53 dependent. |
format | Online Article Text |
id | pubmed-6270821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62708212019-01-07 ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells Despaigne, Angel A. Recio Da Silva, Jeferson G. da Costa, Pryscila R. dos Santos, Raquel G. Beraldo, Heloisa Molecules Article 2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl(2)] (1) and [Cu(H2BzMe)Cl(2)] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 proved to be more active than the corresponding hydrazones against U87, but not against T98 cells. Compound 1 induced higher levels of ROS than H2AcMe in both glioma cell lines. H2AcMe and 1 induced lower levels of ROS in MRC5 than in U87 cells. Compound 2 induced lower levels of ROS in MRC5 than in T98 cells. The cytotoxic effect of 1 in U87 cells could be related to its ability to provoke the release of ROS, suggesting that the cytotoxicity of 1 might be somehow p53 dependent. MDPI 2014-10-27 /pmc/articles/PMC6270821/ /pubmed/25350363 http://dx.doi.org/10.3390/molecules191117202 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Despaigne, Angel A. Recio Da Silva, Jeferson G. da Costa, Pryscila R. dos Santos, Raquel G. Beraldo, Heloisa ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells |
title | ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells |
title_full | ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells |
title_fullStr | ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells |
title_full_unstemmed | ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells |
title_short | ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells |
title_sort | ros-mediated cytotoxic effect of copper(ii) hydrazone complexes against human glioma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270821/ https://www.ncbi.nlm.nih.gov/pubmed/25350363 http://dx.doi.org/10.3390/molecules191117202 |
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