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ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells

2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl(2)] (1) and [Cu(H2BzMe)Cl(2)] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 pro...

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Autores principales: Despaigne, Angel A. Recio, Da Silva, Jeferson G., da Costa, Pryscila R., dos Santos, Raquel G., Beraldo, Heloisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270821/
https://www.ncbi.nlm.nih.gov/pubmed/25350363
http://dx.doi.org/10.3390/molecules191117202
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author Despaigne, Angel A. Recio
Da Silva, Jeferson G.
da Costa, Pryscila R.
dos Santos, Raquel G.
Beraldo, Heloisa
author_facet Despaigne, Angel A. Recio
Da Silva, Jeferson G.
da Costa, Pryscila R.
dos Santos, Raquel G.
Beraldo, Heloisa
author_sort Despaigne, Angel A. Recio
collection PubMed
description 2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl(2)] (1) and [Cu(H2BzMe)Cl(2)] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 proved to be more active than the corresponding hydrazones against U87, but not against T98 cells. Compound 1 induced higher levels of ROS than H2AcMe in both glioma cell lines. H2AcMe and 1 induced lower levels of ROS in MRC5 than in U87 cells. Compound 2 induced lower levels of ROS in MRC5 than in T98 cells. The cytotoxic effect of 1 in U87 cells could be related to its ability to provoke the release of ROS, suggesting that the cytotoxicity of 1 might be somehow p53 dependent.
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spelling pubmed-62708212019-01-07 ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells Despaigne, Angel A. Recio Da Silva, Jeferson G. da Costa, Pryscila R. dos Santos, Raquel G. Beraldo, Heloisa Molecules Article 2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl(2)] (1) and [Cu(H2BzMe)Cl(2)] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 proved to be more active than the corresponding hydrazones against U87, but not against T98 cells. Compound 1 induced higher levels of ROS than H2AcMe in both glioma cell lines. H2AcMe and 1 induced lower levels of ROS in MRC5 than in U87 cells. Compound 2 induced lower levels of ROS in MRC5 than in T98 cells. The cytotoxic effect of 1 in U87 cells could be related to its ability to provoke the release of ROS, suggesting that the cytotoxicity of 1 might be somehow p53 dependent. MDPI 2014-10-27 /pmc/articles/PMC6270821/ /pubmed/25350363 http://dx.doi.org/10.3390/molecules191117202 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Despaigne, Angel A. Recio
Da Silva, Jeferson G.
da Costa, Pryscila R.
dos Santos, Raquel G.
Beraldo, Heloisa
ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
title ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
title_full ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
title_fullStr ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
title_full_unstemmed ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
title_short ROS-Mediated Cytotoxic Effect of Copper(II) Hydrazone Complexes against Human Glioma Cells
title_sort ros-mediated cytotoxic effect of copper(ii) hydrazone complexes against human glioma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270821/
https://www.ncbi.nlm.nih.gov/pubmed/25350363
http://dx.doi.org/10.3390/molecules191117202
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