Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code. In particular, we engineered the intracellular biosynthes...

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Detalles Bibliográficos
Autores principales: Ma, Ying, Biava, Hernán, Contestabile, Roberto, Budisa, Nediljko, di Salvo, Martino Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271081/
https://www.ncbi.nlm.nih.gov/pubmed/24434673
http://dx.doi.org/10.3390/molecules19011004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271081/
https://www.ncbi.nlm.nih.gov/pubmed/24434673
http://dx.doi.org/10.3390/molecules19011004

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