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Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code. In particular, we engineered the intracellular biosynthes...

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Detalles Bibliográficos
Autores principales:	Ma, Ying, Biava, Hernán, Contestabile, Roberto, Budisa, Nediljko, di Salvo, Martino Luigi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271081/ https://www.ncbi.nlm.nih.gov/pubmed/24434673 http://dx.doi.org/10.3390/molecules19011004

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