Cargando…
High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces
We report here the in vitro selection of DNA aptamers for electric eel acetylcholinesterase (AChE). One selected aptamer sequence (R15/19) has a high affinity towards the enzyme (K(d) = 157 ± 42 pM). Characterization of the aptamer showed its binding is not affected by low ionic strength (~20 mM), h...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271157/ https://www.ncbi.nlm.nih.gov/pubmed/24756130 http://dx.doi.org/10.3390/molecules19044986 |