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High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces

We report here the in vitro selection of DNA aptamers for electric eel acetylcholinesterase (AChE). One selected aptamer sequence (R15/19) has a high affinity towards the enzyme (K(d) = 157 ± 42 pM). Characterization of the aptamer showed its binding is not affected by low ionic strength (~20 mM), h...

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Detalles Bibliográficos
Autores principales:	Chumphukam, Orada, Le, Thao T., Cass, Anthony E. G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271157/ https://www.ncbi.nlm.nih.gov/pubmed/24756130 http://dx.doi.org/10.3390/molecules19044986

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