Cargando…
Curcumin Reverse Methicillin Resistance in Staphylococcus aureus
Curcumin, a natural polyphenolic flavonoid extracted from the rhizome of Curcuma longa L., was shown to possess superior potency to resensitize methicillin-resistant Staphylococcus aureus (MRSA) to antibiotics. Previous studies have shown the synergistic activity of curcumin with β-lactam and quinol...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271166/ https://www.ncbi.nlm.nih.gov/pubmed/25389660 http://dx.doi.org/10.3390/molecules191118283 |
_version_ | 1783376865760641024 |
---|---|
author | Mun, Su-Hyun Kim, Sung-Bae Kong, Ryong Choi, Jang-Gi Kim, Youn-Chul Shin, Dong-Won Kang, Ok-Hwa Kwon, Dong-Yeul |
author_facet | Mun, Su-Hyun Kim, Sung-Bae Kong, Ryong Choi, Jang-Gi Kim, Youn-Chul Shin, Dong-Won Kang, Ok-Hwa Kwon, Dong-Yeul |
author_sort | Mun, Su-Hyun |
collection | PubMed |
description | Curcumin, a natural polyphenolic flavonoid extracted from the rhizome of Curcuma longa L., was shown to possess superior potency to resensitize methicillin-resistant Staphylococcus aureus (MRSA) to antibiotics. Previous studies have shown the synergistic activity of curcumin with β-lactam and quinolone antibiotics. Further, to understand the anti-MRSA mechanism of curcumin, we investigated the potentiated effect of curcumin by its interaction in diverse conditions. The mechanism of anti-MRSA action of curcumin was analyzed by the viability assay in the presence of detergents, ATPase inhibitors and peptidoglycan (PGN) from S. aureus, and the PBP2a protein level was analyzed by western blotting. The morphological changes in the curcumin-treated MRSA strains were investigated by transmission electron microscopy (TEM). We analyzed increased susceptibility to MRSA isolates in the presence of curcumin. The optical densities at 600 nm (OD(600)) of the suspensions treated with the combinations of curcumin with triton X-100 and Tris were reduced to 63% and 59%, respectively, compared to curcumin without treatment. N,N'-dicyclohexylcarbodiimide (DCCD) and sodium azide (NaN(3)) were reduced to 94% and 55%, respectively. When peptidoglycan (PGN) from S. aureus was combined with curcumin, PGN (0–125 μg/mL) gradually blocked the antibacterial activity of curcumin (125 μg/mL); however, at a concentration of 125 µg/mL PGN, it did not completely block curcumin. Curcumin has a significant effect on the protein level of PBP2a. The TEM images of MRSA showed damage of the cell wall, disruption of the cytoplasmic contents, broken cell membrane and cell lysis after the treatment of curcumin. These data indicate a remarkable antibacterial effect of curcumin, with membrane permeability enhancers and ATPase inhibitors, and curcumin did not directly bind to PGN on the cell wall. Further, the antimicrobial action of curcumin involved in the PBP2a-mediated resistance mechanism was investigated. |
format | Online Article Text |
id | pubmed-6271166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62711662019-01-07 Curcumin Reverse Methicillin Resistance in Staphylococcus aureus Mun, Su-Hyun Kim, Sung-Bae Kong, Ryong Choi, Jang-Gi Kim, Youn-Chul Shin, Dong-Won Kang, Ok-Hwa Kwon, Dong-Yeul Molecules Article Curcumin, a natural polyphenolic flavonoid extracted from the rhizome of Curcuma longa L., was shown to possess superior potency to resensitize methicillin-resistant Staphylococcus aureus (MRSA) to antibiotics. Previous studies have shown the synergistic activity of curcumin with β-lactam and quinolone antibiotics. Further, to understand the anti-MRSA mechanism of curcumin, we investigated the potentiated effect of curcumin by its interaction in diverse conditions. The mechanism of anti-MRSA action of curcumin was analyzed by the viability assay in the presence of detergents, ATPase inhibitors and peptidoglycan (PGN) from S. aureus, and the PBP2a protein level was analyzed by western blotting. The morphological changes in the curcumin-treated MRSA strains were investigated by transmission electron microscopy (TEM). We analyzed increased susceptibility to MRSA isolates in the presence of curcumin. The optical densities at 600 nm (OD(600)) of the suspensions treated with the combinations of curcumin with triton X-100 and Tris were reduced to 63% and 59%, respectively, compared to curcumin without treatment. N,N'-dicyclohexylcarbodiimide (DCCD) and sodium azide (NaN(3)) were reduced to 94% and 55%, respectively. When peptidoglycan (PGN) from S. aureus was combined with curcumin, PGN (0–125 μg/mL) gradually blocked the antibacterial activity of curcumin (125 μg/mL); however, at a concentration of 125 µg/mL PGN, it did not completely block curcumin. Curcumin has a significant effect on the protein level of PBP2a. The TEM images of MRSA showed damage of the cell wall, disruption of the cytoplasmic contents, broken cell membrane and cell lysis after the treatment of curcumin. These data indicate a remarkable antibacterial effect of curcumin, with membrane permeability enhancers and ATPase inhibitors, and curcumin did not directly bind to PGN on the cell wall. Further, the antimicrobial action of curcumin involved in the PBP2a-mediated resistance mechanism was investigated. MDPI 2014-11-10 /pmc/articles/PMC6271166/ /pubmed/25389660 http://dx.doi.org/10.3390/molecules191118283 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mun, Su-Hyun Kim, Sung-Bae Kong, Ryong Choi, Jang-Gi Kim, Youn-Chul Shin, Dong-Won Kang, Ok-Hwa Kwon, Dong-Yeul Curcumin Reverse Methicillin Resistance in Staphylococcus aureus |
title | Curcumin Reverse Methicillin Resistance in Staphylococcus aureus |
title_full | Curcumin Reverse Methicillin Resistance in Staphylococcus aureus |
title_fullStr | Curcumin Reverse Methicillin Resistance in Staphylococcus aureus |
title_full_unstemmed | Curcumin Reverse Methicillin Resistance in Staphylococcus aureus |
title_short | Curcumin Reverse Methicillin Resistance in Staphylococcus aureus |
title_sort | curcumin reverse methicillin resistance in staphylococcus aureus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271166/ https://www.ncbi.nlm.nih.gov/pubmed/25389660 http://dx.doi.org/10.3390/molecules191118283 |
work_keys_str_mv | AT munsuhyun curcuminreversemethicillinresistanceinstaphylococcusaureus AT kimsungbae curcuminreversemethicillinresistanceinstaphylococcusaureus AT kongryong curcuminreversemethicillinresistanceinstaphylococcusaureus AT choijanggi curcuminreversemethicillinresistanceinstaphylococcusaureus AT kimyounchul curcuminreversemethicillinresistanceinstaphylococcusaureus AT shindongwon curcuminreversemethicillinresistanceinstaphylococcusaureus AT kangokhwa curcuminreversemethicillinresistanceinstaphylococcusaureus AT kwondongyeul curcuminreversemethicillinresistanceinstaphylococcusaureus |