Bioconversion of Rebaudioside I from Rebaudioside A

To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one...

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Autores principales: Prakash, Indra, Bunders, Cynthia, Devkota, Krishna P., Charan, Romila D., Ramirez, Catherine, Snyder, Tara M., Priedemann, Christopher, Markosyan, Avetik, Jarrin, Cyrille, Halle, Robert Ter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271207/
https://www.ncbi.nlm.nih.gov/pubmed/25353385
http://dx.doi.org/10.3390/molecules191117345
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author Prakash, Indra
Bunders, Cynthia
Devkota, Krishna P.
Charan, Romila D.
Ramirez, Catherine
Snyder, Tara M.
Priedemann, Christopher
Markosyan, Avetik
Jarrin, Cyrille
Halle, Robert Ter
author_facet Prakash, Indra
Bunders, Cynthia
Devkota, Krishna P.
Charan, Romila D.
Ramirez, Catherine
Snyder, Tara M.
Priedemann, Christopher
Markosyan, Avetik
Jarrin, Cyrille
Halle, Robert Ter
author_sort Prakash, Indra
collection PubMed
description To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy ((1)H, (13)C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.
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spelling pubmed-62712072019-01-07 Bioconversion of Rebaudioside I from Rebaudioside A Prakash, Indra Bunders, Cynthia Devkota, Krishna P. Charan, Romila D. Ramirez, Catherine Snyder, Tara M. Priedemann, Christopher Markosyan, Avetik Jarrin, Cyrille Halle, Robert Ter Molecules Article To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy ((1)H, (13)C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I. MDPI 2014-10-28 /pmc/articles/PMC6271207/ /pubmed/25353385 http://dx.doi.org/10.3390/molecules191117345 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Prakash, Indra
Bunders, Cynthia
Devkota, Krishna P.
Charan, Romila D.
Ramirez, Catherine
Snyder, Tara M.
Priedemann, Christopher
Markosyan, Avetik
Jarrin, Cyrille
Halle, Robert Ter
Bioconversion of Rebaudioside I from Rebaudioside A
title Bioconversion of Rebaudioside I from Rebaudioside A
title_full Bioconversion of Rebaudioside I from Rebaudioside A
title_fullStr Bioconversion of Rebaudioside I from Rebaudioside A
title_full_unstemmed Bioconversion of Rebaudioside I from Rebaudioside A
title_short Bioconversion of Rebaudioside I from Rebaudioside A
title_sort bioconversion of rebaudioside i from rebaudioside a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271207/
https://www.ncbi.nlm.nih.gov/pubmed/25353385
http://dx.doi.org/10.3390/molecules191117345
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