Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities

Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink...

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Detalles Bibliográficos
Autores principales: Akisawa, Takuya, Ishizawa, Yuki, Nagatsugi, Fumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272193/
https://www.ncbi.nlm.nih.gov/pubmed/25781072
http://dx.doi.org/10.3390/molecules20034708
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author Akisawa, Takuya
Ishizawa, Yuki
Nagatsugi, Fumi
author_facet Akisawa, Takuya
Ishizawa, Yuki
Nagatsugi, Fumi
author_sort Akisawa, Takuya
collection PubMed
description Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA.
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spelling pubmed-62721932018-12-31 Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities Akisawa, Takuya Ishizawa, Yuki Nagatsugi, Fumi Molecules Article Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA. MDPI 2015-03-13 /pmc/articles/PMC6272193/ /pubmed/25781072 http://dx.doi.org/10.3390/molecules20034708 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akisawa, Takuya
Ishizawa, Yuki
Nagatsugi, Fumi
Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities
title Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities
title_full Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities
title_fullStr Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities
title_full_unstemmed Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities
title_short Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities
title_sort synthesis of peptide nucleic acids containing a crosslinking agent and evaluation of their reactivities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272193/
https://www.ncbi.nlm.nih.gov/pubmed/25781072
http://dx.doi.org/10.3390/molecules20034708
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AT ishizawayuki synthesisofpeptidenucleicacidscontainingacrosslinkingagentandevaluationoftheirreactivities
AT nagatsugifumi synthesisofpeptidenucleicacidscontainingacrosslinkingagentandevaluationoftheirreactivities

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