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Purification and Immobilization of the Recombinant Brassica oleracea Chlorophyllase 1 (BoCLH1) on DIAION(®)CR11 as Potential Biocatalyst for the Production of Chlorophyllide and Phytol

Recombinant Brassica oleracea chlorophyllase 1 (BoCLH1) with a protein molecular weight of 38.63 kDa was successfully expressed in E. coli and could catalyze chlorophyll (Chl) hydrolysis to chlorophyllide and phytol in vitro. In this study, we used DIAION(®)CR11, a highly porous cross-linked polysty...

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Detalles Bibliográficos
Autores principales: Chou, Yi-Li, Ko, Chia-Yun, Chen, Long-Fang O., Yen, Chih-Chung, Shaw, Jei-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272265/
https://www.ncbi.nlm.nih.gov/pubmed/25719743
http://dx.doi.org/10.3390/molecules20033744