Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization

A novel β-galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o-...

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Detalles Bibliográficos
Autores principales: Benavente, Rocio, Pessela, Benevides C., Curiel, Jose Antonio, de las Rivas, Blanca, Muñoz, Rosario, Guisán, Jose Manuel, Mancheño, Jose M., Cardelle-Cobas, Alejandra, Ruiz-Matute, Ana I., Corzo, Nieves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272498/
https://www.ncbi.nlm.nih.gov/pubmed/25942370
http://dx.doi.org/10.3390/molecules20057874

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272498/
https://www.ncbi.nlm.nih.gov/pubmed/25942370
http://dx.doi.org/10.3390/molecules20057874

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