Cargando…

A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages

Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed th...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Zhi-Hang, Liang, Zeng-Enni, Wu, Jing, Yi, Jin-E, Chen, Xiao-Jun, Sun, Zhi-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273091/
https://www.ncbi.nlm.nih.gov/pubmed/27706063
http://dx.doi.org/10.3390/molecules21101317
_version_ 1783377304828772352
author Yuan, Zhi-Hang
Liang, Zeng-Enni
Wu, Jing
Yi, Jin-E
Chen, Xiao-Jun
Sun, Zhi-Liang
author_facet Yuan, Zhi-Hang
Liang, Zeng-Enni
Wu, Jing
Yi, Jin-E
Chen, Xiao-Jun
Sun, Zhi-Liang
author_sort Yuan, Zhi-Hang
collection PubMed
description Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis.
format Online
Article
Text
id pubmed-6273091
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62730912018-12-28 A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages Yuan, Zhi-Hang Liang, Zeng-Enni Wu, Jing Yi, Jin-E Chen, Xiao-Jun Sun, Zhi-Liang Molecules Article Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis. MDPI 2016-10-01 /pmc/articles/PMC6273091/ /pubmed/27706063 http://dx.doi.org/10.3390/molecules21101317 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuan, Zhi-Hang
Liang, Zeng-Enni
Wu, Jing
Yi, Jin-E
Chen, Xiao-Jun
Sun, Zhi-Liang
A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
title A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
title_full A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
title_fullStr A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
title_full_unstemmed A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
title_short A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
title_sort potential mechanism for the anti-apoptotic property of koumine involving mitochondrial pathway in lps-mediated raw 264.7 macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273091/
https://www.ncbi.nlm.nih.gov/pubmed/27706063
http://dx.doi.org/10.3390/molecules21101317
work_keys_str_mv AT yuanzhihang apotentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT liangzengenni apotentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT wujing apotentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT yijine apotentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT chenxiaojun apotentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT sunzhiliang apotentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT yuanzhihang potentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT liangzengenni potentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT wujing potentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT yijine potentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT chenxiaojun potentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages
AT sunzhiliang potentialmechanismfortheantiapoptoticpropertyofkoumineinvolvingmitochondrialpathwayinlpsmediatedraw2647macrophages