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Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi
Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be furt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273529/ https://www.ncbi.nlm.nih.gov/pubmed/27775633 http://dx.doi.org/10.3390/molecules21101395 |
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author | Wang, Xiao-Ling Ding, Zhong-Yang Liu, Gao-Qiang Yang, Hailong Zhou, Guo-Ying |
author_facet | Wang, Xiao-Ling Ding, Zhong-Yang Liu, Gao-Qiang Yang, Hailong Zhou, Guo-Ying |
author_sort | Wang, Xiao-Ling |
collection | PubMed |
description | Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. In this study, enhanced TA yield and productivity were attained with G. lingzhi using response surface methodology. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 °C. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. Additionally, the triterpene acid extract (TAE) from G. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract. |
format | Online Article Text |
id | pubmed-6273529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62735292018-12-28 Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi Wang, Xiao-Ling Ding, Zhong-Yang Liu, Gao-Qiang Yang, Hailong Zhou, Guo-Ying Molecules Article Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. In this study, enhanced TA yield and productivity were attained with G. lingzhi using response surface methodology. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 °C. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. Additionally, the triterpene acid extract (TAE) from G. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract. MDPI 2016-10-20 /pmc/articles/PMC6273529/ /pubmed/27775633 http://dx.doi.org/10.3390/molecules21101395 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Xiao-Ling Ding, Zhong-Yang Liu, Gao-Qiang Yang, Hailong Zhou, Guo-Ying Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi |
title | Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi |
title_full | Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi |
title_fullStr | Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi |
title_full_unstemmed | Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi |
title_short | Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi |
title_sort | improved production and antitumor properties of triterpene acids from submerged culture of ganoderma lingzhi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273529/ https://www.ncbi.nlm.nih.gov/pubmed/27775633 http://dx.doi.org/10.3390/molecules21101395 |
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