Site-Specific, Covalent Immobilization of Dehalogenase ST2570 Catalyzed by Formylglycine-Generating Enzymes and Its Application in Batch and Semi-Continuous Flow Reactors

Formylglycine-generating enzymes can selectively recognize and oxidize cysteine residues within the sulfatase sub motif at the terminus of proteins to form aldehyde-bearing formylglycine (FGly) residues, and are normally used in protein labeling. In this study, an aldehyde tag was introduced to prot...

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Detalles Bibliográficos
Autores principales: Jian, Hui, Wang, Yingwu, Bai, Yan, Li, Rong, Gao, Renjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273756/
https://www.ncbi.nlm.nih.gov/pubmed/27409601
http://dx.doi.org/10.3390/molecules21070895

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273756/
https://www.ncbi.nlm.nih.gov/pubmed/27409601
http://dx.doi.org/10.3390/molecules21070895

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