Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes

The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X(1)), extraction ti...

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Autores principales: Liu, Hongzhi, Jiang, Nan, Liu, Li, Sheng, Xiaojing, Shi, Aimin, Hu, Hui, Yang, Ying, Wang, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274344/
https://www.ncbi.nlm.nih.gov/pubmed/27258246
http://dx.doi.org/10.3390/molecules21060716
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author Liu, Hongzhi
Jiang, Nan
Liu, Li
Sheng, Xiaojing
Shi, Aimin
Hu, Hui
Yang, Ying
Wang, Qiang
author_facet Liu, Hongzhi
Jiang, Nan
Liu, Li
Sheng, Xiaojing
Shi, Aimin
Hu, Hui
Yang, Ying
Wang, Qiang
author_sort Liu, Hongzhi
collection PubMed
description The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X(1)), extraction time (X(2)) and ratio of water to raw material (X(3)). The optimum conditions were 85 °C, 3 h and 20:1 (mL/g) respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 10(5) Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide.
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spelling pubmed-62743442018-12-28 Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes Liu, Hongzhi Jiang, Nan Liu, Li Sheng, Xiaojing Shi, Aimin Hu, Hui Yang, Ying Wang, Qiang Molecules Article The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X(1)), extraction time (X(2)) and ratio of water to raw material (X(3)). The optimum conditions were 85 °C, 3 h and 20:1 (mL/g) respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 10(5) Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide. MDPI 2016-06-01 /pmc/articles/PMC6274344/ /pubmed/27258246 http://dx.doi.org/10.3390/molecules21060716 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Hongzhi
Jiang, Nan
Liu, Li
Sheng, Xiaojing
Shi, Aimin
Hu, Hui
Yang, Ying
Wang, Qiang
Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
title Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
title_full Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
title_fullStr Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
title_full_unstemmed Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
title_short Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes
title_sort extraction, purification and primary characterization of polysaccharides from defatted peanut (arachis hypogaea) cakes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274344/
https://www.ncbi.nlm.nih.gov/pubmed/27258246
http://dx.doi.org/10.3390/molecules21060716
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