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A Simple Technique for Three-Dimensional Imaging and Segmentation of Brain Vasculature Using Fast Free-of-Acrylamide Clearing Tissue in Murine

OBJECTIVE: Fast Free-of-Acrylamide Clearing Tissue (FACT) is a recently developed protocol for the whole tissue three-dimensional (3D) imaging. The FACT protocol clears lipids using sodium dodecyl sulfate (SDS) to increase the penetration of light and reflection of fluorescent signals from the depth...

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Detalles Bibliográficos
Autores principales: Khoradmehr, Arezoo, Mazaheri, Fahime, Anvari, Morteza, Tamadon, Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275429/
https://www.ncbi.nlm.nih.gov/pubmed/30507088
http://dx.doi.org/10.22074/cellj.2019.5684