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Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activi...

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Autores principales: Olchowik, Ewa, Lotkowski, Karol, Mavlyanov, Saidmukhtar, Abdullajanova, Nodira, Ionov, Maksim, Bryszewska, Maria, Zamaraeva, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275782/
https://www.ncbi.nlm.nih.gov/pubmed/22491984
http://dx.doi.org/10.2478/s11658-012-0014-7
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author Olchowik, Ewa
Lotkowski, Karol
Mavlyanov, Saidmukhtar
Abdullajanova, Nodira
Ionov, Maksim
Bryszewska, Maria
Zamaraeva, Maria
author_facet Olchowik, Ewa
Lotkowski, Karol
Mavlyanov, Saidmukhtar
Abdullajanova, Nodira
Ionov, Maksim
Bryszewska, Maria
Zamaraeva, Maria
author_sort Olchowik, Ewa
collection PubMed
description Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO(−) or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.
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spelling pubmed-62757822018-12-10 Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.) Olchowik, Ewa Lotkowski, Karol Mavlyanov, Saidmukhtar Abdullajanova, Nodira Ionov, Maksim Bryszewska, Maria Zamaraeva, Maria Cell Mol Biol Lett Short Communication Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO(−) or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes. SP Versita 2012-04-10 /pmc/articles/PMC6275782/ /pubmed/22491984 http://dx.doi.org/10.2478/s11658-012-0014-7 Text en © © Versita Warsaw and Springer-Verlag Wien 2012
spellingShingle Short Communication
Olchowik, Ewa
Lotkowski, Karol
Mavlyanov, Saidmukhtar
Abdullajanova, Nodira
Ionov, Maksim
Bryszewska, Maria
Zamaraeva, Maria
Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)
title Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)
title_full Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)
title_fullStr Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)
title_full_unstemmed Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)
title_short Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)
title_sort stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (rhus typhina l.) and grape seeds (vitis vinifera l.)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275782/
https://www.ncbi.nlm.nih.gov/pubmed/22491984
http://dx.doi.org/10.2478/s11658-012-0014-7
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