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Gene trapping: An antibody-dependent approach for verifying integration in your favorite gene

Gene trapping is used to introduce genome-wide insertional mutations in embryonic stem cells. Determining the integration site is based on highthroughput PCR, which has inevitable possibilities for mistakes, thus necessitating clone verification prior to the generation of mutant mice. Here, we propo...

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Detalles Bibliográficos
Autores principales: Gorelik, Anna, Sapir, Tamar, Reiner, Orly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275795/
https://www.ncbi.nlm.nih.gov/pubmed/18618084
http://dx.doi.org/10.2478/s11658-008-0028-3