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Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation

The evolutionarily-conserved protein phosphatase 1 (PP1) plays a central role in dephosphorylation of phosphoproteins during the M phase of the cell cycle. We demonstrate here that the PP1 inhibitor inhibitor-2 protein (Inh-2) induces an M-phase arrest in Xenopus cycling egg extracts. Interestingly,...

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Autores principales: Khandani, Arian, Mohtashami, Mahmood, Camirand, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275968/
https://www.ncbi.nlm.nih.gov/pubmed/21956525
http://dx.doi.org/10.2478/s11658-011-0030-z
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author Khandani, Arian
Mohtashami, Mahmood
Camirand, Anne
author_facet Khandani, Arian
Mohtashami, Mahmood
Camirand, Anne
author_sort Khandani, Arian
collection PubMed
description The evolutionarily-conserved protein phosphatase 1 (PP1) plays a central role in dephosphorylation of phosphoproteins during the M phase of the cell cycle. We demonstrate here that the PP1 inhibitor inhibitor-2 protein (Inh-2) induces an M-phase arrest in Xenopus cycling egg extracts. Interestingly, the characteristics of this M-phase arrest are similar to those of mitogen-activated protein kinase (p42MAPK)-induced M-phase arrest. This prompted us to investigate whether Inh-2-induced M-phase arrest was dependent on activation of the p42MAPK pathway. We demonstrate here that MAPK activity is required for Inh-2-induced M-phase arrest, as inhibition of MAPK by PD98059 allowed cycling extracts to exit M phase, despite the presence of Inh-2. We next investigated whether Inh-2 phosphorylation by the MAPK pathway was required to induce an M-phase arrest. We discovered that while p90Rsk (a MAPK protein required for M-phase arrest) is able to phosphorylate Inh-2, this phosphorylation is not required for Inh-2 function. Overall, our results suggest a novel mechanism linking p42MAPK and PP1 pathways during M phase of the cell cycle. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.2478/s11658-011-0030-z and is accessible for authorized users.
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spelling pubmed-62759682018-12-10 Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation Khandani, Arian Mohtashami, Mahmood Camirand, Anne Cell Mol Biol Lett Research Article The evolutionarily-conserved protein phosphatase 1 (PP1) plays a central role in dephosphorylation of phosphoproteins during the M phase of the cell cycle. We demonstrate here that the PP1 inhibitor inhibitor-2 protein (Inh-2) induces an M-phase arrest in Xenopus cycling egg extracts. Interestingly, the characteristics of this M-phase arrest are similar to those of mitogen-activated protein kinase (p42MAPK)-induced M-phase arrest. This prompted us to investigate whether Inh-2-induced M-phase arrest was dependent on activation of the p42MAPK pathway. We demonstrate here that MAPK activity is required for Inh-2-induced M-phase arrest, as inhibition of MAPK by PD98059 allowed cycling extracts to exit M phase, despite the presence of Inh-2. We next investigated whether Inh-2 phosphorylation by the MAPK pathway was required to induce an M-phase arrest. We discovered that while p90Rsk (a MAPK protein required for M-phase arrest) is able to phosphorylate Inh-2, this phosphorylation is not required for Inh-2 function. Overall, our results suggest a novel mechanism linking p42MAPK and PP1 pathways during M phase of the cell cycle. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.2478/s11658-011-0030-z and is accessible for authorized users. SP Versita 2011-09-26 /pmc/articles/PMC6275968/ /pubmed/21956525 http://dx.doi.org/10.2478/s11658-011-0030-z Text en © © Versita Warsaw and Springer-Verlag Wien 2011
spellingShingle Research Article
Khandani, Arian
Mohtashami, Mahmood
Camirand, Anne
Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
title Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
title_full Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
title_fullStr Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
title_full_unstemmed Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
title_short Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
title_sort inhibitor-2 induced m-phase arrest in xenopus cycling egg extracts is dependent on mapk activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275968/
https://www.ncbi.nlm.nih.gov/pubmed/21956525
http://dx.doi.org/10.2478/s11658-011-0030-z
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