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Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
The evolutionarily-conserved protein phosphatase 1 (PP1) plays a central role in dephosphorylation of phosphoproteins during the M phase of the cell cycle. We demonstrate here that the PP1 inhibitor inhibitor-2 protein (Inh-2) induces an M-phase arrest in Xenopus cycling egg extracts. Interestingly,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SP Versita
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275968/ https://www.ncbi.nlm.nih.gov/pubmed/21956525 http://dx.doi.org/10.2478/s11658-011-0030-z |
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author | Khandani, Arian Mohtashami, Mahmood Camirand, Anne |
author_facet | Khandani, Arian Mohtashami, Mahmood Camirand, Anne |
author_sort | Khandani, Arian |
collection | PubMed |
description | The evolutionarily-conserved protein phosphatase 1 (PP1) plays a central role in dephosphorylation of phosphoproteins during the M phase of the cell cycle. We demonstrate here that the PP1 inhibitor inhibitor-2 protein (Inh-2) induces an M-phase arrest in Xenopus cycling egg extracts. Interestingly, the characteristics of this M-phase arrest are similar to those of mitogen-activated protein kinase (p42MAPK)-induced M-phase arrest. This prompted us to investigate whether Inh-2-induced M-phase arrest was dependent on activation of the p42MAPK pathway. We demonstrate here that MAPK activity is required for Inh-2-induced M-phase arrest, as inhibition of MAPK by PD98059 allowed cycling extracts to exit M phase, despite the presence of Inh-2. We next investigated whether Inh-2 phosphorylation by the MAPK pathway was required to induce an M-phase arrest. We discovered that while p90Rsk (a MAPK protein required for M-phase arrest) is able to phosphorylate Inh-2, this phosphorylation is not required for Inh-2 function. Overall, our results suggest a novel mechanism linking p42MAPK and PP1 pathways during M phase of the cell cycle. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.2478/s11658-011-0030-z and is accessible for authorized users. |
format | Online Article Text |
id | pubmed-6275968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SP Versita |
record_format | MEDLINE/PubMed |
spelling | pubmed-62759682018-12-10 Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation Khandani, Arian Mohtashami, Mahmood Camirand, Anne Cell Mol Biol Lett Research Article The evolutionarily-conserved protein phosphatase 1 (PP1) plays a central role in dephosphorylation of phosphoproteins during the M phase of the cell cycle. We demonstrate here that the PP1 inhibitor inhibitor-2 protein (Inh-2) induces an M-phase arrest in Xenopus cycling egg extracts. Interestingly, the characteristics of this M-phase arrest are similar to those of mitogen-activated protein kinase (p42MAPK)-induced M-phase arrest. This prompted us to investigate whether Inh-2-induced M-phase arrest was dependent on activation of the p42MAPK pathway. We demonstrate here that MAPK activity is required for Inh-2-induced M-phase arrest, as inhibition of MAPK by PD98059 allowed cycling extracts to exit M phase, despite the presence of Inh-2. We next investigated whether Inh-2 phosphorylation by the MAPK pathway was required to induce an M-phase arrest. We discovered that while p90Rsk (a MAPK protein required for M-phase arrest) is able to phosphorylate Inh-2, this phosphorylation is not required for Inh-2 function. Overall, our results suggest a novel mechanism linking p42MAPK and PP1 pathways during M phase of the cell cycle. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.2478/s11658-011-0030-z and is accessible for authorized users. SP Versita 2011-09-26 /pmc/articles/PMC6275968/ /pubmed/21956525 http://dx.doi.org/10.2478/s11658-011-0030-z Text en © © Versita Warsaw and Springer-Verlag Wien 2011 |
spellingShingle | Research Article Khandani, Arian Mohtashami, Mahmood Camirand, Anne Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation |
title | Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation |
title_full | Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation |
title_fullStr | Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation |
title_full_unstemmed | Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation |
title_short | Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation |
title_sort | inhibitor-2 induced m-phase arrest in xenopus cycling egg extracts is dependent on mapk activation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275968/ https://www.ncbi.nlm.nih.gov/pubmed/21956525 http://dx.doi.org/10.2478/s11658-011-0030-z |
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