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Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity
We investigated the effects of Rho-associated kinase (ROCK) on migration and cytoskeletal organization in primary human osteoblasts and Saos-2 human osteosarcoma cells. Both cell types were exposed to two different ROCK inhibitors, Y-27632 and HA-1077. In the improved motility assay used in the pres...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SP Versita
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275969/ https://www.ncbi.nlm.nih.gov/pubmed/21394446 http://dx.doi.org/10.2478/s11658-011-0006-z |
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author | Zhang, Xuejiao Li, Cheng Gao, Huiling Nabeka, Hiroaki Shimokawa, Tetsuya Wakisaka, Hiroyuki Matsuda, Seiji Kobayashi, Naoto |
author_facet | Zhang, Xuejiao Li, Cheng Gao, Huiling Nabeka, Hiroaki Shimokawa, Tetsuya Wakisaka, Hiroyuki Matsuda, Seiji Kobayashi, Naoto |
author_sort | Zhang, Xuejiao |
collection | PubMed |
description | We investigated the effects of Rho-associated kinase (ROCK) on migration and cytoskeletal organization in primary human osteoblasts and Saos-2 human osteosarcoma cells. Both cell types were exposed to two different ROCK inhibitors, Y-27632 and HA-1077. In the improved motility assay used in the present study, Y-27632 and HA-1077 significantly increased the migration of both osteoblasts and osteosarcoma cells on plastic in a dose-dependent and reversible manner. Fluorescent images showed that cells of both types cultured with Y-27632 or HA-1077 exhibited a stellate appearance, with poor assembly of stress fibers and focal contacts. Western blotting showed that ROCK inhibitors reduced myosin light chain (MLC) phosphorylation within 5 min without affecting overall myosin light-chain protein levels. Inhibition of ROCK activity is thought to enhance the migration of human osteoblasts through reorganization of the actin cytoskeleton and regulation of myosin activity. ROCK inhibitors may be potentially useful as anabolic agents to enhance the biocompatibility of bone and joint prostheses. |
format | Online Article Text |
id | pubmed-6275969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SP Versita |
record_format | MEDLINE/PubMed |
spelling | pubmed-62759692018-12-10 Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity Zhang, Xuejiao Li, Cheng Gao, Huiling Nabeka, Hiroaki Shimokawa, Tetsuya Wakisaka, Hiroyuki Matsuda, Seiji Kobayashi, Naoto Cell Mol Biol Lett Research Article We investigated the effects of Rho-associated kinase (ROCK) on migration and cytoskeletal organization in primary human osteoblasts and Saos-2 human osteosarcoma cells. Both cell types were exposed to two different ROCK inhibitors, Y-27632 and HA-1077. In the improved motility assay used in the present study, Y-27632 and HA-1077 significantly increased the migration of both osteoblasts and osteosarcoma cells on plastic in a dose-dependent and reversible manner. Fluorescent images showed that cells of both types cultured with Y-27632 or HA-1077 exhibited a stellate appearance, with poor assembly of stress fibers and focal contacts. Western blotting showed that ROCK inhibitors reduced myosin light chain (MLC) phosphorylation within 5 min without affecting overall myosin light-chain protein levels. Inhibition of ROCK activity is thought to enhance the migration of human osteoblasts through reorganization of the actin cytoskeleton and regulation of myosin activity. ROCK inhibitors may be potentially useful as anabolic agents to enhance the biocompatibility of bone and joint prostheses. SP Versita 2011-03-09 /pmc/articles/PMC6275969/ /pubmed/21394446 http://dx.doi.org/10.2478/s11658-011-0006-z Text en © © Versita Warsaw and Springer-Verlag Wien 2011 |
spellingShingle | Research Article Zhang, Xuejiao Li, Cheng Gao, Huiling Nabeka, Hiroaki Shimokawa, Tetsuya Wakisaka, Hiroyuki Matsuda, Seiji Kobayashi, Naoto Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
title | Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
title_full | Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
title_fullStr | Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
title_full_unstemmed | Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
title_short | Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
title_sort | rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275969/ https://www.ncbi.nlm.nih.gov/pubmed/21394446 http://dx.doi.org/10.2478/s11658-011-0006-z |
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