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Optimised LAMP allows single copy detection of 35Sp and NOSt in transgenic maize using Bioluminescent Assay in Real Time (BART)

Loop-mediated amplification (LAMP) has been widely used to amplify and hence detect nucleic acid target sequences from various pathogens, viruses and genetic modifications. Two distinct types of primer are required for LAMP; hairpin-forming LAMP and displacement. High specificity arises from this us...

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Detalles Bibliográficos
Autores principales: Hardinge, Patrick, Kiddle, Guy, Tisi, Laurence, Murray, James A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279926/
https://www.ncbi.nlm.nih.gov/pubmed/30514874
http://dx.doi.org/10.1038/s41598-018-36207-4