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Optimised LAMP allows single copy detection of 35Sp and NOSt in transgenic maize using Bioluminescent Assay in Real Time (BART)
Loop-mediated amplification (LAMP) has been widely used to amplify and hence detect nucleic acid target sequences from various pathogens, viruses and genetic modifications. Two distinct types of primer are required for LAMP; hairpin-forming LAMP and displacement. High specificity arises from this us...
Autores principales: | Hardinge, Patrick, Kiddle, Guy, Tisi, Laurence, Murray, James A. H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279926/ https://www.ncbi.nlm.nih.gov/pubmed/30514874 http://dx.doi.org/10.1038/s41598-018-36207-4 |
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