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DIG-seq: a genome-wide CRISPR off-target profiling method using chromatin DNA
To investigate whether and how CRISPR-Cas9 on-target and off-target activities are affected by chromatin in eukaryotic cells, we first identified a series of identical endogenous DNA sequences present in both open and closed chromatin regions and then measured mutation frequencies at these sites in...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Cold Spring Harbor Laboratory Press
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280750/ https://www.ncbi.nlm.nih.gov/pubmed/30413470 http://dx.doi.org/10.1101/gr.236620.118 |