An accurate TMT-based approach to quantify and model lysine susceptibility to conjugation via N-hydroxysuccinimide esters in a monoclonal antibody

Conjugation of small molecules to proteins through N-hydroxysuccinimide (NHS) esters results in a random distribution of small molecules on lysine residues and the protein N-terminus. While mass spectrometry methods have improved characterization of these protein conjugates, it remains a challenge t...

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Detalles Bibliográficos
Autores principales: Hill, Jennifer J., Tremblay, Tammy-Lynn, Corbeil, Christopher R., Purisima, Enrico O., Sulea, Traian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281681/
https://www.ncbi.nlm.nih.gov/pubmed/30518942
http://dx.doi.org/10.1038/s41598-018-35924-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281681/
https://www.ncbi.nlm.nih.gov/pubmed/30518942
http://dx.doi.org/10.1038/s41598-018-35924-0

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