High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

BACKGROUND: Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated by protein factors, and this regulation can impact on the levels of miRNAs and m...

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Detalles Bibliográficos
Autores principales: Bouvette, Jonathan, Korkut, Dursun Nizam, Fouillen, Aurélien, Amellah, Soumiya, Nanci, Antonio, Durocher, Yves, Omichinski, James G., Legault, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282390/
https://www.ncbi.nlm.nih.gov/pubmed/30522464
http://dx.doi.org/10.1186/s12896-018-0485-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282390/
https://www.ncbi.nlm.nih.gov/pubmed/30522464
http://dx.doi.org/10.1186/s12896-018-0485-3

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