A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand–nucleic acid structural selectivity

We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic acid binders containing a variety chemical scaffolds. We used a combina...

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Detalles Bibliográficos
Autores principales: del Villar-Guerra, Rafael, Gray, Robert D, Trent, John O, Chaires, Jonathan B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283418/
https://www.ncbi.nlm.nih.gov/pubmed/29361140
http://dx.doi.org/10.1093/nar/gky019

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283418/
https://www.ncbi.nlm.nih.gov/pubmed/29361140
http://dx.doi.org/10.1093/nar/gky019

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