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In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep

Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against...

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Autores principales: Silva Soares, Suelen Carolina, de Lima, Gilmar Cotrin, Carlos Laurentiz, Antonio, Féboli, Aline, dos Anjos, Luciano Alves, de Paula Carlis, Matheus Souza, da Silva Filardi, Rosimeire, da Silva de Laurentiz, Rosangela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine, Cairo University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286422/
https://www.ncbi.nlm.nih.gov/pubmed/30564603
http://dx.doi.org/10.1016/j.ijvsm.2018.11.005
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author Silva Soares, Suelen Carolina
de Lima, Gilmar Cotrin
Carlos Laurentiz, Antonio
Féboli, Aline
dos Anjos, Luciano Alves
de Paula Carlis, Matheus Souza
da Silva Filardi, Rosimeire
da Silva de Laurentiz, Rosangela
author_facet Silva Soares, Suelen Carolina
de Lima, Gilmar Cotrin
Carlos Laurentiz, Antonio
Féboli, Aline
dos Anjos, Luciano Alves
de Paula Carlis, Matheus Souza
da Silva Filardi, Rosimeire
da Silva de Laurentiz, Rosangela
author_sort Silva Soares, Suelen Carolina
collection PubMed
description Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD(50) values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep.
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spelling pubmed-62864222018-12-18 In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep Silva Soares, Suelen Carolina de Lima, Gilmar Cotrin Carlos Laurentiz, Antonio Féboli, Aline dos Anjos, Luciano Alves de Paula Carlis, Matheus Souza da Silva Filardi, Rosimeire da Silva de Laurentiz, Rosangela Int J Vet Sci Med Short Communication Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD(50) values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep. Faculty of Veterinary Medicine, Cairo University 2018-11-18 /pmc/articles/PMC6286422/ /pubmed/30564603 http://dx.doi.org/10.1016/j.ijvsm.2018.11.005 Text en © 2018 Faculty of Veterinary Medicine, Cairo University. Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Silva Soares, Suelen Carolina
de Lima, Gilmar Cotrin
Carlos Laurentiz, Antonio
Féboli, Aline
dos Anjos, Luciano Alves
de Paula Carlis, Matheus Souza
da Silva Filardi, Rosimeire
da Silva de Laurentiz, Rosangela
In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
title In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
title_full In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
title_fullStr In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
title_full_unstemmed In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
title_short In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
title_sort in vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286422/
https://www.ncbi.nlm.nih.gov/pubmed/30564603
http://dx.doi.org/10.1016/j.ijvsm.2018.11.005
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