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Algorithms for automated detection of hook effect-bearing amplification curves

Amplification curves from quantitative Real-Time PCR experiments typically exhibit a sigmoidal shape. They can roughly be divided into a ground or baseline phase, an exponential amplification phase, a linear phase and finally a plateau phase, where in the latter, the PCR product concentration no lon...

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Detalles Bibliográficos
Autores principales: Burdukiewicz, Michał, Spiess, Andrej-Nikolai, Blagodatskikh, Konstantin A., Lehmann, Werner, Schierack, Peter, Rödiger, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287529/
https://www.ncbi.nlm.nih.gov/pubmed/30560061
http://dx.doi.org/10.1016/j.bdq.2018.08.001