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Essential role of polymerases for assay performance – Impact of polymerase replacement in a well-established assay

The quantitative real-time polymerase chain reaction (qPCR) is one of the most commonly molecular methods used today. It is central to numerous assays that have since been developed and described around its optimization. The Listeria monocytogenes prfA qPCR assay has been studied in great detail and...

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Detalles Bibliográficos
Autores principales: Witte, Anna Kristina, Sickha, Romana, Mester, Patrick, Fister, Susanne, Schoder, Dagmar, Rossmanith, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287537/
https://www.ncbi.nlm.nih.gov/pubmed/30560063
http://dx.doi.org/10.1016/j.bdq.2018.10.002