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GoFish: A versatile nested PCR strategy for environmental DNA assays for marine vertebrates

Here we describe GoFish, a strategy for single-species environmental DNA (eDNA) presence/absence assays using nested PCR. The assays amplify a mitochondrial 12S rDNA segment with vertebrate metabarcoding primers, followed by nested PCR with M13-tailed, species-specific primers. Sanger sequencing con...

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Detalles Bibliográficos
Autores principales: Stoeckle, Mark Y., Das Mishu, Mithun, Charlop-Powers, Zachary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289459/
https://www.ncbi.nlm.nih.gov/pubmed/30533051
http://dx.doi.org/10.1371/journal.pone.0198717