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Novel high-throughput screening method using quantitative PCR to determine the antimicrobial susceptibility of Orientia tsutsugamushi clinical isolates

OBJECTIVES: To develop a method to enable the large-scale antimicrobial susceptibility screening of Orientia tsutsugamushi clinical isolates, using one timepoint and one concentration of antibiotics to considerably speed up the time to result. METHODS: Growth, harvesting, multiplicity of infection (...

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Detalles Bibliográficos
Autores principales: Phuklia, Weerawat, Panyanivong, Phonepasith, Sengdetka, Davanh, Sonthayanon, Piengchan, Newton, Paul N, Paris, Daniel H, Day, Nicholas P J, Dittrich, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293087/
https://www.ncbi.nlm.nih.gov/pubmed/30295746
http://dx.doi.org/10.1093/jac/dky402