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Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM
Glioblastoma multiforme (GBM) remains a cancer with a poor prognosis and few effective therapeutic options. Successful medical management of GBM is limited by the restricted access of drugs to the central nervous system (CNS) caused by the blood brain barrier (BBB). We previously showed that a subse...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294248/ https://www.ncbi.nlm.nih.gov/pubmed/30546006 http://dx.doi.org/10.1038/s41419-018-1195-4 |
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author | Przystal, Justyna Magdalena Hajji, Nabil Khozoie, Combiz Renziehausen, Alexander Zeng, Qingyu Abaitua, Fernando Hajitou, Amin Suwan, Keittisak Want, Elizabeth Bomalaski, John Szlosarek, Peter O’Neill, Kevin Crook, Tim Syed, Nelofer |
author_facet | Przystal, Justyna Magdalena Hajji, Nabil Khozoie, Combiz Renziehausen, Alexander Zeng, Qingyu Abaitua, Fernando Hajitou, Amin Suwan, Keittisak Want, Elizabeth Bomalaski, John Szlosarek, Peter O’Neill, Kevin Crook, Tim Syed, Nelofer |
author_sort | Przystal, Justyna Magdalena |
collection | PubMed |
description | Glioblastoma multiforme (GBM) remains a cancer with a poor prognosis and few effective therapeutic options. Successful medical management of GBM is limited by the restricted access of drugs to the central nervous system (CNS) caused by the blood brain barrier (BBB). We previously showed that a subset of GBM are arginine auxotrophic because of transcriptional silencing of ASS1 and/or ASL and are sensitive to pegylated arginine deiminase (ADI-PEG20). However, it is unknown whether depletion of arginine in peripheral blood in vivo has therapeutic activity against intracranial disease. In the present work, we describe the efficacy of ADI-PEG20 in an intracranial model of human GBM in which tumour growth and regression are assessed in real time by measurement of luciferase activity. Animals bearing intracranial human GBM tumours of varying ASS status were treated with ADI-PEG20 alone or in combination with temozolomide and monitored for tumour growth and regression. Monotherapy ADI-PEG20 significantly reduces the intracranial growth of ASS1 negative GBM and extends survival of mice carrying ASS1 negative GBM without obvious toxicity. The combination of ADI-PEG20 with temozolomide (TMZ) demonstrates enhanced effects in both ASS1 negative and ASS1 positive backgrounds.Our data provide proof of principle for a therapeutic strategy for GBM using peripheral blood arginine depletion that does not require BBB passage of drug and is well tolerated. The ability of ADI-PEG20 to cytoreduce GBM and enhance the effects of temozolomide argues strongly for its early clinical evaluation in the treatment of GBM. |
format | Online Article Text |
id | pubmed-6294248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62942482018-12-17 Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM Przystal, Justyna Magdalena Hajji, Nabil Khozoie, Combiz Renziehausen, Alexander Zeng, Qingyu Abaitua, Fernando Hajitou, Amin Suwan, Keittisak Want, Elizabeth Bomalaski, John Szlosarek, Peter O’Neill, Kevin Crook, Tim Syed, Nelofer Cell Death Dis Article Glioblastoma multiforme (GBM) remains a cancer with a poor prognosis and few effective therapeutic options. Successful medical management of GBM is limited by the restricted access of drugs to the central nervous system (CNS) caused by the blood brain barrier (BBB). We previously showed that a subset of GBM are arginine auxotrophic because of transcriptional silencing of ASS1 and/or ASL and are sensitive to pegylated arginine deiminase (ADI-PEG20). However, it is unknown whether depletion of arginine in peripheral blood in vivo has therapeutic activity against intracranial disease. In the present work, we describe the efficacy of ADI-PEG20 in an intracranial model of human GBM in which tumour growth and regression are assessed in real time by measurement of luciferase activity. Animals bearing intracranial human GBM tumours of varying ASS status were treated with ADI-PEG20 alone or in combination with temozolomide and monitored for tumour growth and regression. Monotherapy ADI-PEG20 significantly reduces the intracranial growth of ASS1 negative GBM and extends survival of mice carrying ASS1 negative GBM without obvious toxicity. The combination of ADI-PEG20 with temozolomide (TMZ) demonstrates enhanced effects in both ASS1 negative and ASS1 positive backgrounds.Our data provide proof of principle for a therapeutic strategy for GBM using peripheral blood arginine depletion that does not require BBB passage of drug and is well tolerated. The ability of ADI-PEG20 to cytoreduce GBM and enhance the effects of temozolomide argues strongly for its early clinical evaluation in the treatment of GBM. Nature Publishing Group UK 2018-12-13 /pmc/articles/PMC6294248/ /pubmed/30546006 http://dx.doi.org/10.1038/s41419-018-1195-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Przystal, Justyna Magdalena Hajji, Nabil Khozoie, Combiz Renziehausen, Alexander Zeng, Qingyu Abaitua, Fernando Hajitou, Amin Suwan, Keittisak Want, Elizabeth Bomalaski, John Szlosarek, Peter O’Neill, Kevin Crook, Tim Syed, Nelofer Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM |
title | Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM |
title_full | Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM |
title_fullStr | Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM |
title_full_unstemmed | Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM |
title_short | Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM |
title_sort | efficacy of arginine depletion by adi-peg20 in an intracranial model of gbm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294248/ https://www.ncbi.nlm.nih.gov/pubmed/30546006 http://dx.doi.org/10.1038/s41419-018-1195-4 |
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