Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes

In the present study, we attempted to control the pH profile of the catalytic activity of the industrially relevant alkaline protease KP-43, by incorporating 3-nitro-l-tyrosine and 3-chloro-l-tyrosine at and near the catalytic site. Thirty KP-43 variants containing these non-natural amino acids at t...

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Detalles Bibliográficos
Autores principales: Osamura, Tatsuya, Okuda, Mitsuyoshi, Yamaguchi, Atsushi, Ohtake, Kazumasa, Sakamoto, Kensaku, Takimura, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295607/
https://www.ncbi.nlm.nih.gov/pubmed/30582013
http://dx.doi.org/10.1016/j.bbrep.2018.12.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295607/
https://www.ncbi.nlm.nih.gov/pubmed/30582013
http://dx.doi.org/10.1016/j.bbrep.2018.12.001

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