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Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes
In the present study, we attempted to control the pH profile of the catalytic activity of the industrially relevant alkaline protease KP-43, by incorporating 3-nitro-l-tyrosine and 3-chloro-l-tyrosine at and near the catalytic site. Thirty KP-43 variants containing these non-natural amino acids at t...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295607/ https://www.ncbi.nlm.nih.gov/pubmed/30582013 http://dx.doi.org/10.1016/j.bbrep.2018.12.001 |